[SMLE] Plasma lncRNA LOC338963 and mRNA AP3B2 are upregulated in paraneoplastic Lambert-Eaton Myasthenic Syndrome

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[SMLE] Plasma lncRNA LOC338963 and mRNA AP3B2 are upregulated in paraneoplastic Lambert-Eaton Myasthenic Syndrome

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Muscle Nerve. 2022 May 4. doi: 10.1002/mus.27571. Online ahead of print.

ABSTRACT

INTRODUCTION/AIMS: Lambert-Eaton myasthenic syndrome (LEMS) is an autoimmune neuromuscular junction disorder. Long non-coding RNA (LncRNA) can regulate the expression of mRNA and is involved in the development of autoimmune diseases, but few genetic studies are available. This study aimed to explore the lncRNA and mRNA changes of LEMS.

METHODS: Plasma lncRNA and mRNA expression profiles of three LEMS patients with small cell lung cancer (SCLC) and three matched healthy controls were analyzed by microarray. Differentially expressed lncRNAs and adjacent mRNAs were jointly analyzed, and candidates were verified by quantitative real-time polymerase chain reaction (qRT-PCR). The identified genes were subsequently evaluated in 9, 8, and 4 patients with paraneoplastic LEMS, non-tumor LEMS, and SCLC, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to determine possible functions.

RESULTS: A total of 320 lncRNA and 168 mRNAs were differentially expressed in the three LEMS with SCLC compared to healthy controls. Among them, lncRNA LOC338963 and its neighboring mRNA AP3B2 were upregulated jointly, which was confirmed by qRT-PCR. qRT-PCR revealed significant upregulation of the 2 genes in patients with paraneoplastic LEMS compared to non-tumor LEMS or SCLC. GO analysis of AP3B2 identified the enrichment terms anterograde synaptic vesicle transport and establishment of synaptic vesicle localization. KEEG analysis showed that AP3B2 was enriched in lysosomal pathways.

DISCUSSION: LOC338963 and AP3B2 were upregulated in patients with paraneoplastic LEMS, suggesting their involvement in pathogenesis. These genes could be targets for exploring the pathomechanism of paraneoplastic LEMS. This article is protected by copyright. All rights reserved.

PMID:35508598 | DOI:10.1002/mus.27571


Source: https://pubmed.ncbi.nlm.nih.gov/3550859 ... 4&v=2.17.6
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