[Pubmed] A novel circ_0018483/miR-4310/ERRFI1 ceRNA axis in myasthenia gravis

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J Neuroimmunol. 2026 Apr 21;417:578938. doi: 10.1016/j.jneuroim.2026.578938. Online ahead of print.

ABSTRACT

BACKGROUND: Myasthenia gravis (MG) is one of the most prevalent disorder of the neuromuscular junction (NMJ). Non-coding RNAs regulate myoblast proliferation and differentiation.

METHODS: The differential expression of miRNAs was analysed by using RNA microarrays. Protein levels and gene expression were analysed by using Western blotting and quantitative real-time polymerase chain reaction. The interactions of miR-4310 with circ_0018483 or ERBB receptor feedback inhibitor 1 (ERRFI1) were assessed using dual-luciferase reporter assays. The expression and aggregation of acetylcholine receptors (AChRs) were observed and analysed by using immunofluorescence.

RESULTS: The expression of miR-4310 was downregulated in the whole blood of patients. circ_0018483 exhibited a sponge effect on miR-4310, and the inhibition of miR-4310 weakened AChR function. Additionally, miR-4310 targeted ERRFI1 and circ_0018483, and circ_0018483 affected the expression and aggregation of AChR by regulating miR-4310 and ERRFI1. circ_0018483 regulates the expression of ERRFI1 by targeting miR-4310, which in turn affects AChR expression and aggregation at the NMJ.

CONCLUSIONS: We confirmed a novel circ_0018483/miR-4310/ERRFI1 ceRNA axis in MG. Our findings suggest novel AChR regulation and MG molecular mechanisms research avenues.

PMID:42048741 | DOI:10.1016/j.jneuroim.2026.578938


Source: https://pubmed.ncbi.nlm.nih.gov/4204874 ... t6+133c1fe